Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20020] to Neurofilament heavy polypeptide - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free
    See all Neurofilament heavy polypeptide primary antibodies

  • Description

    Rabbit monoclonal [EPR20020] to Neurofilament heavy polypeptide - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse and rat cerebellum and brain lysates; Neuro-2a and SH-SY5Y whole cell lysates; Human midbrain lysate. IHC-P: Human cerebrum and cerebellum tissues; Mouse cerebellum tissue; Rat cerebral cortex tissue. IHC-Fr: Mouse hippocampus tissue; Rat cerebral cortex tissue. ICC/IF: Neuro-2a cells.

  • General notes

    Ab223139 is the carrier-free version of ab207176. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab223139 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on the normal Human cerebrum tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207176).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)
    Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Positive staining on mouse hippocampus is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207176).

  • Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)
    Immunohistochemistry (Frozen sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebral cortex tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Positive staining on rat cerebral cortex is observed.

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207176).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

    Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Cytoplasm staining on Human cerebellum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207176).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    Cytoplasm staining on mouse cerebellum is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207176).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

    Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on neurons of the rat cerebral cortex is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207176).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Neurofilament heavy polypeptide with ab207176 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: No staining on human kidney.

    Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207176).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)
    Immunocytochemistry/ Immunofluorescence - Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling Neurofilament heavy polypeptide with ab207176 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing cytoplasmic staining on Neuro-2a cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207176).

  • Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)
    Anti-Neurofilament heavy polypeptide antibody [EPR20020] - BSA and Azide free (ab223139)

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