Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP673Y] to Neurofilament heavy polypeptide
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-Neurofilament heavy polypeptide antibody [EP673Y]
See all Neurofilament heavy polypeptide primary antibodies
Description

Rabbit monoclonal [EP673Y] to Neurofilament heavy polypeptide
Host species

Rabbit
Specificity

ab40796 recognises Neuofilaments (NF).

Tested Applications & Species

Application	Species
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Neurofilament heavy polypeptide aa 650-750. The exact sequence is proprietary.
Positive control
- WB: Human, mouse and rat brain tissue lysate. IHC-P: Human gliocytoma tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP673Y
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain glyoma tissue labelling

Neurofilament heavy polypeptide with unpurified ab40796.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)

Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution (purified) + Human brain tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 112 kDa
Observed band size: 200-220 kDa
why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)

All lanes : Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 112 kDa
Observed band size: 200-220 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)

Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796) at 1/2000 dilution (unpurified) + Rat brain tissue lysate

Predicted band size: 112 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gliocytoma tissue labelling Neurofilament heavy polypeptide with purified ab40796 at 1/8000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Anti-Neurofilament heavy polypeptide antibody [EP673Y] (ab40796)

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