Anti-MK2 (phospho T334) antibody [MAPKAPK2T334-H2] (ab278705)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MAPKAPK2T334-H2] to MK2 (phospho T334)
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Overview
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Product name
Anti-MK2 (phospho T334) antibody [MAPKAPK2T334-H2]
See all MK2 primary antibodies -
Description
Rabbit monoclonal [MAPKAPK2T334-H2] to MK2 (phospho T334) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide within Human MK2 (phospho T334). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P49137 -
Positive control
- WB: HeLa (treated with UV +TPA) cell extract. Flow cyt: U937 cells (treated with CalA); NIH/3T3 cells (treated with UV).
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 49.88% PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
MAPKAPK2T334-H2 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with CalA (green) using ab278705 at 0.1 µg/mL.
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All lanes : Anti-MK2 (phospho T334) antibody [MAPKAPK2T334-H2] (ab278705) at 0.05 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) untreated, whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with UV+TPA, whole cell lysate
Predicted band size: 46 kDa
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Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) treated with CalA (green) with 10X non-phospho peptide (violet) or phospho-peptide (brown) using ab28705 at 0.1 µg/mL.
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Flow cytometric analysis of NIH/3T3 cells untreated (red) or treated with UV (green) usingab278705 at 0.05 ug/mL, or concentration-matched Rabbit (G9) mAb IgG Isotype Control for cells untreated (black) or treated with UV (blue).
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