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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)

Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [NF421] to Neurofilament heavy polypeptide
  • Suitable for: WB, ICC, IHC-P
  • Reacts with: Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Neurofilament heavy polypeptide antibody [NF421]
    See all Neurofilament heavy polypeptide primary antibodies
  • Description

    Mouse monoclonal [NF421] to Neurofilament heavy polypeptide
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    ICC
    Human
    IHC-P
    Rat
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein corresponding to Human Neurofilament heavy polypeptide aa 1-1026.
    Database link: 4744

  • Positive control

    • IHC-P: Human and rat cerebellum tissues. ICC: HEK-293 cells. WB: Human brain tissue lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified by Protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    NF421
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Axon marker
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebellum tissue labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.

  • Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)
    Immunocytochemistry - Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)

    Immunocytochemistry analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml (green). Membrane stained with Phalloidin (red).

  • Western blot - Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)
    Western blot - Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)
    Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374) at 1 µg/ml + Human brain tissue lysate

    Predicted band size: 112 kDa



    Western blot analysis of human brain tissue lysate labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat cerebellum tissue labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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