Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374)
Key features and details
- Mouse monoclonal [NF421] to Neurofilament heavy polypeptide
- Suitable for: WB, ICC, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-Neurofilament heavy polypeptide antibody [NF421]
See all Neurofilament heavy polypeptide primary antibodies -
Description
Mouse monoclonal [NF421] to Neurofilament heavy polypeptide -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P RatHumanWB Human -
Immunogen
Recombinant full length protein corresponding to Human Neurofilament heavy polypeptide aa 1-1026.
Database link: 4744 -
Positive control
- IHC-P: Human and rat cerebellum tissues. ICC: HEK-293 cells. WB: Human brain tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified by Protein A/G. -
Clonality
Monoclonal -
Clone number
NF421 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cerebellum tissue labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.
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Immunocytochemistry analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml (green). Membrane stained with Phalloidin (red).
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Anti-Neurofilament heavy polypeptide antibody [NF421] (ab187374) at 1 µg/ml + Human brain tissue lysate
Predicted band size: 112 kDaWestern blot analysis of human brain tissue lysate labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat cerebellum tissue labeling Neurofilament heavy polypeptide with ab187374 at 1 ug/ml.