Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
![Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)](https://www.abcam.com/ps/products/7/ab7795/Images/ab7795-265224-anti-neurofilament-heavy-polypeptide-antibody-nf-01-immunohistochemistry.jpg "Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)")
Key features and details
- Mouse monoclonal [NF-01] to Neurofilament heavy polypeptide
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Neurofilament heavy polypeptide antibody [NF-01]
See all Neurofilament heavy polypeptide primary antibodies -
Description
Mouse monoclonal [NF-01] to Neurofilament heavy polypeptide -
Host species
Mouse -
Specificity
This antibody recognizes a phosphorylated epitope on heavy neurofilament protein (210 kDa) of various species. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P Human
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Immunogen
A pellet of pig brain cold-stable proteins after depolymerization of microtubules.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
>95 % pure (by PAGE) -
Clonality
Monoclonal -
Clone number
NF-01 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue sections labelling Neurofilament heavy polypeptide protein with ab7795 at 5 µL.
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![Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)](https://www.abcam.com/ps/products/7/ab7795/Images/ab7795-13258-anti-neurofilament-heavy-polypeptide-antibody-nf-01-flow-cytometry.jpg)
Flow Cytometry - Anti-Neurofilament heavy polypeptide antibody [NF-01] (ab7795)Overlay histogram showing SH-SY5Y cells stained with ab7795 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7795, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
