Anti-MED13L antibody (ab87831)
Key features and details
- Rabbit polyclonal to MED13L
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MED13L antibody
See all MED13L primary antibodies -
Description
Rabbit polyclonal to MED13L -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
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Immunogen
Synthetic peptide within Human MED13L aa 550-600. The exact sequence is proprietary. (GeneID 23389).
Database link: NP_056150.1 -
Positive control
- Whole cell lysate from HeLa cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to MED13L immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MED13L antibody (ab87831)All lanes : Anti-MED13L antibody (ab87831) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Developed using the ECL technique.
Predicted band size: 243 kDa
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED13L antibody (ab87831)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED13L with ab87831 at 1/1000 (1µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-MED13L antibody (ab87831)Detection of MED13L by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87831 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection. Chemiluminescence with an exposure time of 30 seconds.
Lane 1 : Anti-MED13L antibody (ab87831) at 1 µg/ml ((and used for IP at 3ug/mg lysate))
Lane 2 : Anti-MED13L antibody (ab87831) at 1 µg/ml (For IP: Rabbit anti-MED13L antibody only (which recognizes a downstream epitope))
Lane 3 : Anti-MED13L antibody (ab87831) at 1 µg/ml (For IP: Control IgG only)
Lane 1 : For WB: HeLa whole cell lysate at 50ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Lane 2 : For WB: HeLa whole cell lysate at 15ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Lane 3 : For WB: HeLa whole cell lysate at 5ug. For IP: HeLa whole cell lysate (1mg for IP, 20% of IP loaded)
Developed using the ECL technique.
Exposure time: 30 seconds
