Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Procalcitonin antibody [4A6] (ab252821)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [4A6] to Procalcitonin
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Procalcitonin antibody [4A6]
    See all Procalcitonin primary antibodies

  • Description

    Rat monoclonal [4A6] to Procalcitonin

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human Procalcitonin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P01258

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human thyroid medullary carcinoma and thyroid follicular adenocarcinoma. ICC/IF: TT cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    4A6

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Procalcitonin antibody [4A6] (ab252821)
    Immunocytochemistry/ Immunofluorescence - Anti-Procalcitonin antibody [4A6] (ab252821)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized TT cells labelling Procalcitonin with ab252821 at 1/500 dilution, followed by ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in TT cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150157 Goat Anti-rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821)

    Immunohistochemical analysis of paraffin-embedded human thyroid medullary carcinoma tissue labeling Procalcitonin with ab252821 at 1/1000 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human thyroid medullary carcinoma. The section was incubated with ab252821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Procalcitonin with ab252821 at 1/100 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Negative control: No staining on human kidney.

    The section was incubated with ab252821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Procalcitonin antibody [4A6] (ab252821)

    Immunohistochemical analysis of paraffin-embedded human follicular adenocarcinoma of thyroid tissue labeling Procalcitonin with ab252821 at 1/1000 dilution followed by a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882). Positive staining on human follicular adenocarcinoma of thyroid. The section was incubated with ab252821 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-rat IgG H&L (HRP polymer) (ab214882).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-Procalcitonin antibody [4A6] (ab252821)
    Anti-Procalcitonin antibody [4A6] (ab252821)

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