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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Other

Anti-SP17 antibody [EP6496] (ab172626)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-SP17 antibody [EP6496] (ab172626)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit monoclonal [EP6496] to SP17
  • Suitable for: Flow Cyt, ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SP17 antibody [EP6496]
    See all SP17 primary antibodies
  • Description

    Rabbit monoclonal [EP6496] to SP17
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human SP17 aa 1-100. The exact sequence is proprietary.
    Database link: Q15506

  • Positive control

    • WB: Human, mouse and rat testis lysate Flow Cyt: Jurkat cells ICC/IF: Jurkat and K-562 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP6496
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Signal Transduction
    • Second Messenger
    • Nucleotide Messenger
    • cAMP
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other

Images

  • Flow Cytometry - Anti-SP17 antibody [EP6496] (ab172626)
    Flow Cytometry - Anti-SP17 antibody [EP6496] (ab172626)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SP17 with purified ab172626 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    All lanes : Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution (Purified)

    Lane 1 : Rat testis lysates
    Lane 2 : Mouse testis lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 17 kDa
    Observed band size: 22-24 kDa
    why is the actual band size different from the predicted?



    SP17 is triplet protein what have been described in the literature PMID: 9986726.

  • Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)
    Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)

    Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling SP17 with purified ab172626 at 1/100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution (Purified) + Human testis lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 17 kDa
    Observed band size: 22-24 kDa why is the actual band size different from the predicted?



    SP17 is triplet protein what have been described in the literature PMID: 9986726.

  • Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)
    Immunocytochemistry/ Immunofluorescence - Anti-SP17 antibody [EP6496] (ab172626)

    Immunofluorescence analysis of Jurkat cells staining SP17 with ab172626 (unpurified) at 1/100 dilution (green). DAPI nuclear staining (blue).

  • Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    Western blot - Anti-SP17 antibody [EP6496] (ab172626)
    All lanes : Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution (unpurified)

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 17 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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