Anti-SP17 antibody [EP6496] (ab172626)
Key features and details
- Rabbit monoclonal [EP6496] to SP17
- Suitable for: Flow Cyt, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SP17 antibody [EP6496]
See all SP17 primary antibodies -
Description
Rabbit monoclonal [EP6496] to SP17 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanWB MouseRatHuman -
Immunogen
Synthetic peptide within Human SP17 aa 1-100. The exact sequence is proprietary.
Database link: Q15506 -
Positive control
- WB: Human, mouse and rat testis lysate Flow Cyt: Jurkat cells ICC/IF: Jurkat and K-562 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP6496 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling SP17 with purified ab172626 at 1/100 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution (Purified)
Lane 1 : Rat testis lysates
Lane 2 : Mouse testis lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 22-24 kDa why is the actual band size different from the predicted?SP17 is triplet protein what have been described in the literature PMID: 9986726.
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Immunocytochemistry/ Immunofluorescence analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling SP17 with purified ab172626 at 1/100 dilution (10 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution (Purified) + Human testis lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 17 kDa
Observed band size: 22-24 kDa why is the actual band size different from the predicted?SP17 is triplet protein what have been described in the literature PMID: 9986726.
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Immunofluorescence analysis of Jurkat cells staining SP17 with ab172626 (unpurified) at 1/100 dilution (green). DAPI nuclear staining (blue).
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All lanes : Anti-SP17 antibody [EP6496] (ab172626) at 1/1000 dilution (unpurified)
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Human testis lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa