Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SP17 antibody [EPR6497(2)] (ab181079)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6497(2)] to SP17
  • Suitable for: IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-SP17 antibody [EPR6497(2)]
    See all SP17 primary antibodies

  • Description

    Rabbit monoclonal [EPR6497(2)] to SP17

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human testis tissue. IP: Human testis tissue lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] (ab181079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] (ab181079)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling SP17 with purified ab181079 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] (ab181079)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody [EPR6497(2)] (ab181079)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labeling SP17 with unpurified ab181079 at 1/250 dilution. Counterstained with hematoxylin.

  • Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (ab181079)
    Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (ab181079)

    ab181079 (purified) at 1/90 immunoprecipitating SP17 in human testis tissue lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (ab181079)
    Immunoprecipitation - Anti-SP17 antibody [EPR6497(2)] (ab181079)

    Western blot analysis of SP17 in immunoprecipitation pellets from Human testis tissue lysate. Unpurified ab181079 was used at 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500. Lane 2 shows negative control.

  • Anti-SP17 antibody [EPR6497(2)] (ab181079)
    Anti-SP17 antibody [EPR6497(2)] (ab181079)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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