Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Interferon beta antibody (ab85803)

Key features and details

  • Rabbit polyclonal to Interferon beta
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Interferon beta antibody
    See all Interferon beta primary antibodies

  • Description

    Rabbit polyclonal to Interferon beta

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Macaque monkey

  • Immunogen

    Synthetic peptide corresponding to Human Interferon beta aa 1-100 conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Recombinant human Interferon beta protein (ab71475) can be used as a positive control in WB. This antibody gave a positive signal in HepG2 and WI38 whole cell lysates and in the following human tissue lysates: Liver; Lung; Kidney; Spleen.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab85803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 22 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

Images

  • Western blot - Anti-Interferon beta antibody (ab85803)
    Western blot - Anti-Interferon beta antibody (ab85803)
    All lanes : Anti-Interferon beta antibody (ab85803) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : WI-38 whole cell lysate (ab3960)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Lung (Human) Tissue Lysate
    Lane 5 : Human kidney tissue lysate - total protein (ab30203)
    Lane 6 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 110 kDa, 120 kDa, 160 kDa, 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    The Interferon Beta protein sequence contains both a phosphorylation and a glycosylation site (Swissprot). Post-translation modifications could account for the migration of the band at a higher molecular weight than predicted.
  • Immunocytochemistry/ Immunofluorescence - Anti-Interferon beta antibody (ab85803)
    Immunocytochemistry/ Immunofluorescence - Anti-Interferon beta antibody (ab85803)
    ICC/IF image of ab85803 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References (1)

Publishing research using ab85803? Please let us know so that we can cite the reference in this datasheet.

ab85803 has been referenced in 1 publication.

  • Oishi I  et al. Efficient production of human interferon beta in the white of eggs from ovalbumin gene-targeted hens. Sci Rep 8:10203 (2018). PubMed: 29976933

Images

  • Western blot - Anti-Interferon beta antibody (ab85803)
    Western blot - Anti-Interferon beta antibody (ab85803)
    All lanes : Anti-Interferon beta antibody (ab85803) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : WI-38 whole cell lysate (ab3960)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : Lung (Human) Tissue Lysate
    Lane 5 : Human kidney tissue lysate - total protein (ab30203)
    Lane 6 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 22 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 110 kDa, 120 kDa, 160 kDa, 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes


    The Interferon Beta protein sequence contains both a phosphorylation and a glycosylation site (Swissprot). Post-translation modifications could account for the migration of the band at a higher molecular weight than predicted.
  • Immunocytochemistry/ Immunofluorescence - Anti-Interferon beta antibody (ab85803)
    Immunocytochemistry/ Immunofluorescence - Anti-Interferon beta antibody (ab85803)
    ICC/IF image of ab85803 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85803, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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