Anti-MCM7/PRL antibody [EPR1973Y] - BSA and Azide free (ab248716)
![Anti-MCM7/PRL antibody [EPR1973Y] - BSA and Azide free (ab248716)](https://www.abcam.com/ps/products/248/ab248716/Images/ab248716-398288-anti-mcm7-prl-antibody-epr1973y-bsa-and-azide-free-western-blot.jpg "Anti-MCM7/PRL antibody [EPR1973Y] - BSA and Azide free (ab248716)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1973Y] to MCM7/PRL - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-MCM7/PRL antibody [EPR1973Y] - BSA and Azide free
See all MCM7/PRL primary antibodies -
Description
Rabbit monoclonal [EPR1973Y] to MCM7/PRL - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: HeLa cells
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General notes
ab248716 is the carrier-free version of ab134194 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab248716 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as MCM7
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1973Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MCM7/PRL antibody [EPR1973Y] - BSA and Azide free (ab248716)All lanes : Anti-MCM7/PRL antibody [EPR1973Y] (ab134194) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 81 kDaThis data was developed using ab134194, the same antibody clone in a different buffer formulation.
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![Flow Cytometry - Anti-MCM7/PRL antibody [EPR1973Y] - BSA and Azide free (ab248716)](https://www.abcam.com/ps/products/248/ab248716/Images/ab248716-3-anti-mcm7-prl-antibody-epr1973-flowcytometry-hela-human.jpg)
Flow Cytometry - Anti-MCM7/PRL antibody [EPR1973Y] - BSA and Azide free (ab248716)This data was developed using ab134194, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MCM7/PRL with purified ab134194 at 1/600 dilution (1µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue). -
![Anti-MCM7/PRL antibody [EPR1973Y] - BSA and Azide free (ab248716)](https://www.abcam.com/ps/products/248/ab248716/Images/ab248716-2-benefits-of-recombinant-antibodies.png)
Anti-MCM7/PRL antibody [EPR1973Y] - BSA and Azide free (ab248716)
