Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BTK (phospho Y551) antibody [EP267Y] - BSA and Azide free (ab247285)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP267Y] to BTK (phospho Y551) - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-BTK (phospho Y551) antibody [EP267Y] - BSA and Azide free
    See all BTK primary antibodies

  • Description

    Rabbit monoclonal [EP267Y] to BTK (phospho Y551) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody will detect BTK phosphorylation on Tyrosine 551. 

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: Ramos cells

  • General notes

    ab247285 is the carrier-free version of ab40770 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247285 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BTK (phospho Y551) antibody [EP267Y] - BSA and Azide free (ab247285)
    Immunocytochemistry/ Immunofluorescence - Anti-BTK (phospho Y551) antibody [EP267Y] - BSA and Azide free (ab247285)

    Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) treated with pervanadate (1mM 30min) cells labeling BTK with purified ab40770 at 1/500 dilution (2 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40770).

  • Anti-BTK (phospho Y551) antibody [EP267Y] - BSA and Azide free (ab247285)
    Anti-BTK (phospho Y551) antibody [EP267Y] - BSA and Azide free (ab247285)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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