Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19873] to Angiopoietin-like 4/ANGPTL4
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873]
    See all Angiopoietin-like 4/ANGPTL4 primary antibodies

  • Description

    Rabbit monoclonal [EPR19873] to Angiopoietin-like 4/ANGPTL4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A549, SW480, THP-1 and HT-29 whole cell lysates; human kidney and fetal lung lysates. ICC/IF: SW480 and HT-29 cells. Flow Cyt: HT-29 cells.

  • General notes

     This product was previously labelled as Angiopoietin-like 4

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
    Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
    Lanes 1-3 : Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/2000 dilution
    Lanes 4-5 : Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : SW480 (human colorectal adenocarcinoma cell line) whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
    Lane 4 : Human kidney lysate
    Lane 5 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
    Lanes 4-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 45 kDa
    Observed band size: 35,50 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-4: 3 minutes; Lane 5: 5 seconds.

    Angiopoietin-like 4/ANGPTL4 can be cleaved at 161-164 site and this immunogen recognize full length 50KD and C-Terminal fragment 35KD. (PMID:19270337; PMID:10862772).

  • Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
    Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counterstain is DAPI (blue).

    Alpha-Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow Cytometry - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
    Flow Cytometry - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/700 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
    Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
    Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution + HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 45 kDa
    Observed band size: 35,50 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
    Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counterstain is DAPI (blue).

    Alpha-Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)
    Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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