Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µl 281 433 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19873] to Angiopoietin-like 4/ANGPTL4
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873]
See all Angiopoietin-like 4/ANGPTL4 primary antibodies
Description

Rabbit monoclonal [EPR19873] to Angiopoietin-like 4/ANGPTL4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: A549, SW480, THP-1 and HT-29 whole cell lysates; human kidney and fetal lung lysates. ICC/IF: SW480 and HT-29 cells. Flow Cyt: HT-29 cells.
General notes
This product was previously labelled as Angiopoietin-like 4

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19873
Isotype

IgG
Research areas

Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

Lanes 1-3 : Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/2000 dilution
Lanes 4-5 : Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution

Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : SW480 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : Human kidney lysate
Lane 5 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-5 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 45 kDa
Observed band size: 35,50 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-4: 3 minutes; Lane 5: 5 seconds.

Angiopoietin-like 4/ANGPTL4 can be cleaved at 161-164 site and this immunogen recognize full length 50KD and C-Terminal fragment 35KD. (PMID:19270337; PMID:10862772).
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line. The nuclear counterstain is DAPI (blue).

Alpha-Tubulin is detected with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Flow Cytometry - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

Flow cytometric analysis of 4% paraformaldehyde-fixed HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/700 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420) at 1/1000 dilution + HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 45 kDa
Observed band size: 35,50 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT-29 (human colorectal adenocarcinoma cell line) cells labeling Angiopoietin-like 4/ANGPTL4 with ab206420 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT-29 cell line. The nuclear counterstain is DAPI (blue).

Alpha-Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.
Anti-Angiopoietin-like 4/ANGPTL4 antibody [EPR19873] (ab206420)

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