Anti-Interferon beta antibody [EPR22186-266] (ab218229)
![Anti-Interferon beta antibody [EPR22186-266] (ab218229)](https://www.abcam.com/ps/products/218/ab218229/Images/ab218229-316590-anti-interferon-beta-antibody-epr22186-266-immunoprecipitation.jpg "Anti-Interferon beta antibody [EPR22186-266] (ab218229)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22186-266] to Interferon beta
- Suitable for: IP, WB
- Reacts with: Mouse
Overview
-
Product name
Anti-Interferon beta antibody [EPR22186-266]
See all Interferon beta primary antibodies -
Description
Rabbit monoclonal [EPR22186-266] to Interferon beta -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
-
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: RAW 264.7 treated with with 50 µg/ml DMXAA (ab143018) for 7 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate; RAW 264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate. IP: RAW 264.7 treated with 50µg/ml DMXAA (ab143018) for 1h, then together with 300ng/ml brefeldin A (BFA) for another 6h, whole cell lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22186-266 -
Isotype
IgG -
Research areas
Images
-
Immunoprecipitation - Anti-Interferon beta antibody [EPR22186-266] (ab218229)
Interferon beta was immunoprecipitated from 0.35 mg of RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 50μg/ml DMXAA (ab143018) for 1h, then together with 300ng/ml brefeldin A (BFA) for another 6h whole cell lysate with ab218229 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218229 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate 10 μg (Input).
Lane 2: ab218229 IP in RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218229 in RAW 264.7 (treated with DMXAA (ab143018)/BFA) whole cell lysate.Blocking and dilution buffer: 5% NFDM/TBST.
-
![Western blot - Anti-Interferon beta antibody [EPR22186-266] (ab218229)](https://www.abcam.com/ps/products/218/ab218229/Images/ab218229-316589-anti-interferon-beta-antibody-epr22186-266-western-blot.jpg)
Western blot - Anti-Interferon beta antibody [EPR22186-266] (ab218229)All lanes : Anti-Interferon beta antibody [EPR22186-266] (ab218229) at 1/1000 dilution
Lane 1 : Untreated RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : RAW 264.7 treated with 50 µg/ml DMXAA (ab143018) for 7 hours, then with 300 ng/ml Brefeldin A (BFA) added after 1 hour, whole cell lysate
Lane 3 : Untreated RAW 264.7 whole cell lysate
Lane 4 : RAW264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 6 hours, then with 300 ng/ml Brefeldin A (BFA) added after 3 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 22 kDa
Observed band size: 24,26 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1 and 2: 26 seconds; Lanes 3 and 4: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
Interferon beta can be induced to increase the expression by DMXAA or LPS in RAW264.7 cell (PMID: 23027866, PMID: 16020513).
The doublet is likely due to isoforms (PMID: 7352261).
-
![Anti-Interferon beta antibody [EPR22186-266] (ab218229)](https://www.abcam.com/ps/products/218/ab218229/Images/ab218229-3-benefits-of-recombinant-antibodies.png)
Anti-Interferon beta antibody [EPR22186-266] (ab218229)
