Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABCD1/ALD antibody [EPR15929] (ab197013)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15929] to ABCD1/ALD
  • Suitable for: Flow Cyt, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ABCD1/ALD antibody [EPR15929]
    See all ABCD1/ALD primary antibodies

  • Description

    Rabbit monoclonal [EPR15929] to ABCD1/ALD

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2, C6, PC-12, NIH/3T3, JAR and RAW 264.7 cell lysates; Human fetal liver tissue lysate; MCF7 cells.

  • General notes

    Previously labeled as ABCD1. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
    Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
    All lanes : Anti-ABCD1/ALD antibody [EPR15929] (ab197013) at 1/10000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Human fetal liver lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
    Immunocytochemistry/ Immunofluorescence - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ABCD1 / ALD with ab197013 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasm staining on MCF7 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab197013 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
    Flow Cytometry - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)

    ab197013 staining ABCD1 / ALD in the human cell line HeLa (human cervix adenocarcinoma) by flow cytometry. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. The sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
    Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
    Anti-ABCD1/ALD antibody [EPR15929] (ab197013) at 1/10000 dilution + JAR (Human placenta choriocarcinoma cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
    Western blot - Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
    All lanes : Anti-ABCD1/ALD antibody [EPR15929] (ab197013) at 1/10000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-ABCD1/ALD antibody [EPR15929] (ab197013)
    Anti-ABCD1/ALD antibody [EPR15929] (ab197013)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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