Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GLP1R antibody [EPR21819] (ab218532)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21819] to GLP1R
  • Suitable for: IHC-Fr, WB, IHC-P
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-GLP1R antibody [EPR21819]
    See all GLP1R primary antibodies

  • Description

    Rabbit monoclonal [EPR21819] to GLP1R

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    Rat
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Beta-TC-6 whole cell lysate. IHC-P: Mouse and rat pancreas tissue. IHC-Fr: Mouse and rat pancreas tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GLP1R antibody [EPR21819] (ab218532)
    Western blot - Anti-GLP1R antibody [EPR21819] (ab218532)
    Anti-GLP1R antibody [EPR21819] (ab218532) at 1/1000 dilution + Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 66, 46 kDa
    why is the actual band size different from the predicted?


    Exposure time: 84 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST 

    The molecular mass observed is consistent with literature (PMID: 10580424).

    The blot was developed using a high sensitivity ECL substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1R antibody [EPR21819] (ab218532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1R antibody [EPR21819] (ab218532)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue stained for GLP1R using ab218532 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-GLP1R antibody [EPR21819] (ab218532)
    Immunohistochemistry (Frozen sections) - Anti-GLP1R antibody [EPR21819] (ab218532)

    4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized rat pancreas tissue stained for GLP1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in rat pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1R antibody [EPR21819] (ab218532)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLP1R antibody [EPR21819] (ab218532)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue stained for GLP1R using ab218532 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining of mouse pancreatic islet cells (PMID: 24467746) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Performed heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Frozen sections) - Anti-GLP1R antibody [EPR21819] (ab218532)
    Immunohistochemistry (Frozen sections) - Anti-GLP1R antibody [EPR21819] (ab218532)

    4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized mouse pancreas tissue stained for GLP1R (green) using ab218532 at 1/500 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Positive cytoplasmic and membranous staining in mouse pancreatic islet cells (PMID: 18541709; 24695667) is observed. Nuclear counterstained with DAPI.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution.

    Heat-mediated antigen retrieval using Tris-EDTA buffer (pH9.0) (ab94681).

  • Anti-GLP1R antibody [EPR21819] (ab218532)
    Anti-GLP1R antibody [EPR21819] (ab218532)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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