Anti-Cardiac Troponin I antibody [MF4] (ab38210)
![Anti-Cardiac Troponin I antibody [MF4] (ab38210)](https://www.abcam.com/ps/products/38/ab38210/Images/ab38210-212585-anti-cardiac-troponin-i-antibody-mf4-immunohistochemistry.jpg "Anti-Cardiac Troponin I antibody [MF4] (ab38210)")
Key features and details
- Mouse monoclonal [MF4] to Cardiac Troponin I
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Cardiac Troponin I antibody [MF4]
See all Cardiac Troponin I primary antibodies -
Description
Mouse monoclonal [MF4] to Cardiac Troponin I -
Host species
Mouse -
Specificity
This antibody is specific for cardiac Troponin I. It does not react with skeletal muscle cardiac Troponin I. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Free human cardiac Troponin I, native human cardiac Troponin complex, or human cardiac Troponin I peptides.
-
Positive control
- IHC-P: FFPE human heart tissue sections.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MF4 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [MF4] (ab38210)
IHC image of cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab38210, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
