Anti-Cardiac Troponin T antibody [1C11] (ab8295)
![Anti-Cardiac Troponin T antibody [1C11] (ab8295)](https://www.abcam.com/ps/products/8/ab8295/Images/ab8295-318645-anti-cardiac-troponin-t-antibody-1c11-immunohistochemistry.jpg "Anti-Cardiac Troponin T antibody [1C11] (ab8295)")
Key features and details
- Mouse monoclonal [1C11] to Cardiac Troponin T
- Suitable for: ICC, IHC-P, Sandwich ELISA
- Reacts with: Mouse, Dog, Human
- Isotype: IgG1
Overview
-
Product name
Anti-Cardiac Troponin T antibody [1C11]
See all Cardiac Troponin T primary antibodies -
Description
Mouse monoclonal [1C11] to Cardiac Troponin T -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumansELISA Mouse
-
Immunogen
Other Immunogen Type corresponding to Human Cardiac Troponin T aa 171-190.
Database link: P45379 -
Positive control
-
General notes
This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC.
This product was changed from ascites to tissue culture supernatant on 17th October 2017 and product received after this date will be from tissue culture supernatant.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified from TCS -
Primary antibody notes
This antibody detects Troponin T in human cardiac muscle. No cross-reaction with skeletal troponin T, cTnI and TnC. -
Clonality
Monoclonal -
Clone number
1C11 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295) Ye L et al. Decreased Yes-Associated Protein-1 (YAP1) Expression in Pediatric Hearts with Ventricular Septal Defects. PLoS One 10:e0139712 (2015).
Paraffin-embedded Normal human Heart and iVSD heart tissue (were blocked using 10% FBS for 30 min) stained for Cardiac Troponin T (Red) using ab8295 at 1/200 dilution at room temperature for 2 hours. The slides were then incubated with Fluor® 555-conjugated anti-mouse (Abcam, ab150107; 1:1,000 dilution). The nuclear counterstain was DAPI (Blue).
-
![Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (ab8295)](https://www.abcam.com/ps/products/8/ab8295/Images/ab8295-7-anti-cardiac-troponin-t-antibody-immunocytochemistry-ips-myocytes-human-1c11.jpg)
Immunocytochemistry - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Immunofluorescence staining of Cardiac Troponin T using ab8295 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab8295 at 5 µg/mL and ab6046, rabbit polyclonal to beta Tubulin, at 1/1000 dilution. Cells were then incubated with ab150117, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150088, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab8295 gave comparable results using MeOH fixation (100%, 5 min).
-
![Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)](https://www.abcam.com/ps/products/8/ab8295/Images/ab8295-14115-anti-cardiac-troponin-t-antibody-1c11-sandwich-elisa.jpg)
Sandwich ELISA - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Calibration curves for sandwich cTnT fluoroimmunoassay with different animal TnTs as antigen.(dark blue) canine, (blue/grey) human, (grey) mouse, (black) rat. Monoclonal antibodies: capture, ab8295 [clone 1C11], 1µ -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)](https://www.abcam.com/ps/products/8/ab8295/Images/ab8295-14114-anti-cardiac-troponin-t-antibody-1c11-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [1C11] (ab8295)Ab8295 staining human normal heart. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
