Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein (ab198146)
Key features and details
- Expression system: Baculovirus
- Purity: > 96% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag N-Terminus, His tag N-Terminus, His tag N-Terminus, His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein
See all EZH2 + EED + SUZ12 + AEBP2 + RBBP4 proteins and peptides -
Biological activity
0.04 pmol/min/µg
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Purity
> 96 % SDS-PAGE. -
Expression system
Baculovirus -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Predicted molecular weight
86 kDa including tags -
Amino acids
2 to 746 -
Tags
His tag N-Terminus
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Amino Acid Sequence 2
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Species
Human -
Predicted molecular weight
51 kDa including tags -
Amino acids
2 to 441 -
Tags
DDDDK tag N-Terminus
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Amino Acid Sequence 3
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Species
Human -
Predicted molecular weight
87 kDa including tags -
Amino acids
2 to 739 -
Tags
His tag N-Terminus
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Amino Acid Sequence 4
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Species
Human -
Predicted molecular weight
53 kDa including tags -
Amino acids
2 to 503 -
Tags
His tag N-Terminus
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Amino Acid Sequence 5
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Species
Human -
Predicted molecular weight
48 kDa including tags -
Amino acids
2 to 425 -
Tags
His tag N-Terminus
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Preparation and Storage
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Amino Acid Sequence 5
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Species
Human -
Predicted molecular weight
48 kDa including tags -
Amino acids
2 to 425 -
Tags
His tag N-Terminus
Images
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Functional Studies - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein (ab198146)
Specific activity of ab198146.
Assay conditions: 50 µL reaction mix (20 mM phosphate buffer pH 7.4, 0.05% Tween-20, 20 µM S-adenosylmethionine, and 50-200 ng enzyme complex) add to the wells coated with the substrate. Incubate for 1 hour. Add antibody against methylated K27 residue of histone H3, incubate 1 hour. Then, add secondary HRP-labeled antibody and incubate 30 minutes. Finally, add HRP chemiluminescent substrates and read luminesence.
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SDS-PAGE - Recombinant human EZH2 + EED + SUZ12 + AEBP2 + RBBP4 protein (ab198146)4-20% gradient SDS-PAGE Coomassie staining.
Lane 1: 6.2 µg ab198146 enzyme complex
Lane 2: Protein Marker
