Anti-Integrin alpha 9 antibody [EPR9722] (ab140599)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9722] to Integrin alpha 9
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Integrin alpha 9 antibody [EPR9722]
See all Integrin alpha 9 primary antibodies -
Description
Rabbit monoclonal [EPR9722] to Integrin alpha 9 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Mouse -
Immunogen
Synthetic peptide within Human Integrin alpha 9 aa 50-150. The exact sequence is proprietary.
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Positive control
- NIH3T3, A673, HepG2, and A549 cell lysates, Human lung and Human skin tissues
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9722 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Integrin alpha 9 antibody [EPR9722] (ab140599) at 1/1000 dilution
Lane 1 : NIH3T3 cell lysate
Lane 2 : A673 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 114 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 9 antibody [EPR9722] (ab140599)
Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Integrin alpha 9 with ab140599 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Integrin alpha 9 antibody [EPR9722] (ab140599)
Immunohistochemical analysis of paraffin embedded Human skin tissue labelling Integrin alpha 9 with ab140599 antibody at a dilution of 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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