Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9969(B)] to SLC25A13/Citrin
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SLC25A13/Citrin antibody [EPR9969(B)]
    See all SLC25A13/Citrin primary antibodies

  • Description

    Rabbit monoclonal [EPR9969(B)] to SLC25A13/Citrin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SLC25A13/Citrin (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa, Hap1, HepG2 and SH-SY5Y cell lysates; Human fetal liver tissue lysate.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as SLC25A13

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)
    Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)
    All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SLC25A13 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 70 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab167166 was shown to react with SLC25A13 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265668 (knockout cell lysate ab258192) was used. Wild-type HeLa and SLC25A13 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167166 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)
    Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)
    All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/500 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : SLC25A13 knockout HAP1 cell lysate
    Lane 3 : SH-5YSY cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 70 kDa



    Lanes 1 - 3: Merged signal (red and green). Green - ab167166 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab167166 was shown to recognize SLC25A13 when SLC25A13 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A13 knockout samples were subjected to SDS-PAGE. ab167166 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)
    Western blot - Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)
    All lanes : Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166) at 1/1000 dilution

    Lane 1 : Human fetal liver lysates
    Lane 2 : HepG2 cell lysates
    Lane 3 : SH-SY5Y cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 70 kDa

  • Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)
    Anti-SLC25A13/Citrin antibody [EPR9969(B)] (ab167166)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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