Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18115] to Ionotropic Glutamate receptor 2 - BSA and Azide free
  • Suitable for: ICC, WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free
    See all Ionotropic Glutamate receptor 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18115] to Ionotropic Glutamate receptor 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251444 is the carrier-free version of ab206293. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251444 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/5000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate
    Lane 3 : Human fetal brain tissue lysate
    Lane 4 : Rat spleen tissue lysate
    Lane 5 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 6 : Human fetal kidney tissue lysate
    Lane 7 : HT1080 (Human fibrosarcoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 99 kDa
    Observed band size: 99 kDa



    This data was developed using ab206293, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 1 second; Lanes 2-7: 10 seconds.

  • Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    Western blot - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    All lanes : Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] (ab206293) at 1/2000 dilution

    Lane 1 : Human brain tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 5 : Human fetal kidney tissue lysate
    Lane 6 : HT1080 (Human fibrosarcoma cell line) whole cell lysate
    Lane 7 : Mouse heart tissue lysate
    Lane 8 : Rat spleen tissue lysate
    Lane 9 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 & 3-9 : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
    Lane 2 : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 10000 µg

    Predicted band size: 99 kDa
    Observed band size: 99 kDa


    Exposure time: 3 minutes


    This data was developed using ab206293, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    This data was developed using ab206293, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human solitary fibrous tumor tissue labeling Ionotropic Glutamate receptor 2 with ab206293 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on Human solitary fibrous tumor is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    Immunocytochemistry - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    This data was developed using ab206293, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HT1080 (Human fibrosarcoma cell line) cells labeling Ionotropic Glutamate receptor 2 with ab206293 at 1/700 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HT1080 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [EPR18115] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red). The negative controls are as follows:--ve control 1: ab206293 at 1/700 dilution followed by ab150120 at 1/1000 dilution.-ve control 2: ab7291 at 1/1000 dilution followed by ab150077 at 1/1000 dilution.
  • Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    Immunoprecipitation - Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    This data was developed using ab206293, the same antibody clone in a different buffer formulation.Ionotropic Glutamate receptor 2 was immunoprecipitated from 1mg of C6 (Rat glial tumor cell line) whole cell lysate with ab206293 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab206293 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution. Lane 1: C6 whole cell lysate 10µg (Input). Lane 2: ab206293 IP in C6 whole cell lysate. Lane 3:Rabbit IgG, monoclonal [EPR18115] - Isotype Control (ab172730) instead of ab206293 in C6 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 10 seconds.
  • Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)
    Anti-Ionotropic Glutamate receptor 2 antibody [EPR18115] - BSA and Azide free (ab251444)

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