Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)
Key features and details
- Rabbit polyclonal to Ionotropic Glutamate receptor 2
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Ionotropic Glutamate receptor 2 antibody
See all Ionotropic Glutamate receptor 2 primary antibodies -
Description
Rabbit polyclonal to Ionotropic Glutamate receptor 2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Chicken, Human
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse GluR2 .
Read Abcam's proprietary immunogen policy (Peptide available as ab25708.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab20673 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 3 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).Can be blocked with Mouse Ionotropic Glutamate receptor 2 peptide (ab25708). Target
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA2 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-610 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-836 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. - Information by UniProt
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Database links
- Entrez Gene: 2891 Human
- Entrez Gene: 14800 Mouse
- Entrez Gene: 29627 Rat
- Omim: 138247 Human
- SwissProt: P42262 Human
- SwissProt: P23819 Mouse
- SwissProt: P19491 Rat
- Unigene: 32763 Human
see all -
Alternative names
- AMPA 2 antibody
- AMPA selective glutamate receptor 2 antibody
- AMPA-selective glutamate receptor 2 antibody
see all
Images
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Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)Lane 1 : Marker
Lanes 2-5 : Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Cortex (Mouse) Tissue Lysate at 10 µg
Lane 4 : Brain (Mouse) Tissue Lysate at 10 µg withMouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml
Lane 5 : Cortex (Mouse) Tissue Lysate at 10 µg withMouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml
Secondary
Lanes 2-5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands: The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa. -
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.
Protocols
References (19)
ab20673 has been referenced in 19 publications.
- Hu J et al. Constraint-induced movement therapy improves functional recovery after ischemic stroke and its impacts on synaptic plasticity in sensorimotor cortex and hippocampus. Brain Res Bull 160:8-23 (2020). PubMed: 32298779
- Fan J et al. Metformin produces anxiolytic-like effects in rats by facilitating GABAA receptor trafficking to membrane. Br J Pharmacol 176:297-316 (2019). PubMed: 30318707
- Sears SMS et al. Decreased epileptogenesis in mice lacking the System xc- transporter occurs in association with a reduction in AMPA receptor subunit GluA1. Epilepsia Open 4:133-143 (2019). PubMed: 30868123
- Guo B et al. Anterior cingulate cortex dysfunction underlies social deficits in Shank3 mutant mice. Nat Neurosci 22:1223-1234 (2019). PubMed: 31332372
- Wang W et al. Chemogenetic Activation of Prefrontal Cortex Rescues Synaptic and Behavioral Deficits in a Mouse Model of 16p11.2 Deletion Syndrome. J Neurosci 38:5939-5948 (2018). PubMed: 29853627
Images
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Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)Lane 1 : Marker
Lanes 2-5 : Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml
Lane 2 : Brain (Mouse) Tissue Lysate at 10 µg
Lane 3 : Cortex (Mouse) Tissue Lysate at 10 µg
Lane 4 : Brain (Mouse) Tissue Lysate at 10 µg withMouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml
Lane 5 : Cortex (Mouse) Tissue Lysate at 10 µg withMouse Ionotropic Glutamate receptor 2 peptide (ab25708) at 1 µg/ml
Secondary
Lanes 2-5 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands: The band observed at ~50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor. We are unsure as to the identity of the band at ~43KDa. -
Western blot - Anti-Ionotropic Glutamate receptor 2 antibody (ab20673)Anti-Ionotropic Glutamate receptor 2 antibody (ab20673) at 1 µg/ml + Brain (Rat) Tissue Lysate - normal tissue at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 99 kDa
Observed band size: 99 kDa
Additional bands at: 43 kDa. We are unsure as to the identity of these extra bands.The band observed at 50KDa is consistent with the predicted size of Gria2 protein, for which GluR2 is the precursor.
