Ionotropic Agonists Kit (ab120323)
Overview
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Product name
Ionotropic Agonists Kit -
Specificity
A collection of 8 agonists, antagonists and potentiators at AMPA, Kainate and NMDA receptors. Products are freeze-dried to an exact weight so that addition of 1 ml or 0.5 ml of solvent provides a stock solution of 5 mM or 10 mM, respectively. The kit contains (S)-AMPA, (ab120005), (S)-5-Fluorowillardiine hydrochloride (ab120399), Cyclothiazide (ab120061), Aniracetam (ab120316), Kainic acid (ab120100), (S)-5-Iodowillardiine hydrochloride (ab120401), NMDA (ab120052) and D-serine (ab120048). -
Product overview
A collection of 8 agonists, antagonists and potentiators at AMPA, Kainate and NMDA receptors. Products are freeze-dried to an exact weight so that addition of 1 ml or 0.5 ml of solvent provides a stock solution of 5 mM or 10 mM, respectively. The kit contains (S)-AMPA, (ab120005), (S)-5-Fluorowillardiine hydrochloride (ab120399), Cyclothiazide (ab120061), Aniracetam (ab120316), Kainic acid (ab120100), (S)-5-Iodowillardiine hydrochloride (ab120401) , NMDA ab120052) and D-serine (ab120048).
Receptor
Description
AMPA
(S)-AMPA:
AMPA agonist.
Suggested Solvent: Water
AMPA
Cyclothiazide:
Positive allosteric modulator of AMPA receptors. Produces a fast inhibition of AMPA receptor desensitization and a much slower potentiation of the AMPA current.
Suggested Solvent: DMSO
AMPA
Aniracetam:
Nootropic, positive allosteric modulator of AMPA receptors. Slows AMPA receptor deactivation and desensitisation. Antidepressant and anxiolytic in vivo.
Suggested Solvent: DMSO
Kainate
Kainic acid:
Prototypic agonist at the kainate class of ionotropic glutamate receptors. Potent excitant and neurotoxin, used to model epilepsy and neurodegenerative states.
Suggested Solvent: Water
Kainate
NMDA:
Excitotoxic amino acid. Prototypic agonist at the ionotropic NMDA glutamate receptor which is involved in long-term potentiation, ischemia, and epilepsy.
Suggested Solvent: Water
NMDA
D-Serine:
Agonist at the NMDA glycine binding site and the inhibitory post-synaptic glycine receptor.
Suggested Solvent: Water
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Notes
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months.
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
KIT
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit ab120005 - (S)-AMPA 1 vial ab120316 - Aniracetam 1 vial ab120061 - Cyclothiazide 1 vial ab120048 - D-Serine 1 vial ab120100 - Kainic acid (mM/ml), Prototypic kainate receptor agonist 1 vial ab120052 - NMDA 1 vial -
Research areas
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Function
Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. -
Tissue specificity
Widely expressed in brain. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. GRIA1 subfamily. -
Post-translational
modificationsPalmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis. -
Cellular localization
Cell membrane. Endoplasmic reticulum membrane. Cell junction > synapse > postsynaptic cell membrane. Interaction with CACNG2 promotes cell surface expression. - Information by UniProt
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Alternative names
- GLUR 1
- GLUR A
- AMPA 1
see all
