Glutamate Assay Kit (ab83389)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Urine
Overview
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Product name
Glutamate Assay Kit
See all Glutamate kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell Lysate -
Assay type
Quantitative -
Assay time
0h 40m -
Product overview
Glutamate Assay Kit ab83389 provides a sensitive detection method of the glutamate in a variety of samples.
The glutamate assay kit will only measure free glutamate levels but not glutamic acid found in the backbone of peptides or proteins.
In the glutamate assay protocol, the enzyme mix recognizes glutamate as a specific substrate leading to proportional color development.The amount of glutamate can therefore be easily quantified by colorimetric spectrophotometry at OD = 450 nm.
Glutamate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37ºC
- analyze with microplate reader -
Notes
EDTA plasma may not be used with the ab83389 Glutamate Assay Kit. EDTA can interfere with the assay.
Review our Metabolism Assay Guide to learn about assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also about how to assay metabolic function in live cells using your plate reader.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Glutamate Assay Buffer WM 1 x 25ml Glutamate Developer (Lyophilised) Red 1 vial Glutamate Enzyme Mix (Lyophilised) Green 1 vial Glutamate Standard (0.1M) Yellow 1 x 100µl -
Research areas
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Relevance
Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.
Images
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Functional studies - ab83389 Image from Sloniecka M et al., PLoS One 10(7), Fig 5a. Doi: 10.1371/journal.pone.0134157. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Glutamate levels of central and peripheral keratocytes were determined using Glutamate assay kit (ab83389).
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Functional Studies - Glutamate Assay Kit (ab83389)Example of Glutamate standard curve obtained using ab83389
