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Neuroscience Neurotransmitter Transporters Glutamate

Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)

Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11370] to GLUD1 + GLUD2 - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free
  • Description

    Rabbit monoclonal [EPR11370] to GLUD1 + GLUD2 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody immunogen shows 93% identity to human GLUD2, therefore there exists a potential for cross-reactivity.

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver tissue lysate and HepG2 cell lysate. IHC-P: Human heart and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HeLa cells.
  • General notes

    Ab228340 is the carrier-free version of ab168352. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab228340 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11370
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • Glutamate
    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Images

  • Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    Western blot - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    All lanes : Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 61 kDa



    This data was developed using ab168352, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)

    This data was developed using ab168352, the same antibody clone in a different buffer formulation.

    ab168352 showing +ve staining in Human normal pancreas. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    Immunocytochemistry - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)

    This data was developed using ab168352, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of HepG2 cells labeling Glutamate Dehydrogenase with ab168352 at 1/50 dilution.
  • Flow Cytometry - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    Flow Cytometry - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    This data was developed using ab168352, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GLUD1 (red) with ab168352 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    This data was developed using ab168352, the same antibody clone in a different buffer formulation.ab168352 showing +ve staining in Human normal uterus. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    This data was developed using ab168352, the same antibody clone in a different buffer formulation.ab168352 showing +ve staining in Human normal kidney. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    This data was developed using ab168352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    This data was developed using ab168352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
    Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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