Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free (ab228340)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11370] to GLUD1 + GLUD2 - BSA and Azide free
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GLUD1 + GLUD2 antibody [EPR11370] - BSA and Azide free -
Description
Rabbit monoclonal [EPR11370] to GLUD1 + GLUD2 - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody immunogen shows 93% identity to human GLUD2, therefore there exists a potential for cross-reactivity.
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Tested applications
Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal liver tissue lysate and HepG2 cell lysate. IHC-P: Human heart and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HeLa cells.
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General notes
Ab228340 is the carrier-free version of ab168352. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab228340 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11370 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-GLUD1 + GLUD2 antibody [EPR11370] (ab168352) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDaThis data was developed using ab168352, the same antibody clone in a different buffer formulation.
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This data was developed using ab168352, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling GLUD1 (red) with ab168352 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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This data was developed using ab168352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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This data was developed using ab168352, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Glutamate Dehydrogenase with ab168352 at 1/100 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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