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Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735)

Key features and details

  • Mouse monoclonal [322.1.2.2] to Bile Acid Receptor NR1H4 - N-terminal
  • Suitable for: ICC/IF, Flow Cyt, WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal
    See all Bile Acid Receptor NR1H4 primary antibodies

  • Description

    Mouse monoclonal [322.1.2.2] to Bile Acid Receptor NR1H4 - N-terminal

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Bile Acid Receptor NR1H4 aa 1-100 (N terminal) conjugated to ovalbumin. NP_005114.1.
    Database link: Q96RI1-2

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human liver tissue; Bile Acid Receptor NR1H4-transfected Alexander cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735)
    Western blot - Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735)
    All lanes : Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735) at 8 µg/ml

    Lane 1 : Alexander cell lysate
    Lane 2 : Alexander cell lysate transfected with Bile Acid Receptor NR1H4

    Secondary
    All lanes : HRP-labeled goat anti-mouse IgG at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Bile Acid Receptor NR1H4 with ab187735 at 20 µg/ml.

  • Immunocytochemistry/ Immunofluorescence - Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735)
    Immunocytochemistry/ Immunofluorescence - Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735)

    Immunofluorescent analysis of Bile Acid Receptor NR1H4-transfected Alexander cells labeling Bile Acid Receptor NR1H4 with ab187735 at 10 µg/ml. 

  • Flow Cytometry - Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735)
    Flow Cytometry - Anti-Bile Acid Receptor NR1H4 antibody [322.1.2.2] - N-terminal (ab187735)

    Flow Cytometry of Alexander cells.  

    Gray line: control cells
    Black line: cells transfected with FXRa

    Transfection efficiency: 60%

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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