Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)
![Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)](https://www.abcam.com/ps/products/240/ab240379/Images/ab240379-4-ab207777266348207777IFa.jpg "Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20078] to GCLC - BSA and Azide free
- Suitable for: WB, Flow Cyt, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-GCLC antibody [EPR20078] - BSA and Azide free
See all GCLC primary antibodies -
Description
Rabbit monoclonal [EPR20078] to GCLC - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab240379 is the carrier-free version of ab207777. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab240379 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20078 -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling GCLC with ab207777 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on HepG2 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207777).
-
![Immunocytochemistry/ Immunofluorescence - Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)](https://www.abcam.com/ps/products/240/ab240379/Images/ab240379-3-ab207777266347207777IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 (Rat adrenal gland pheochromocytoma cell line) cells labeling GCLC with ab207777 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weak nuclear staining on PC-12 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207777).
-
![Flow Cytometry - Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)](https://www.abcam.com/ps/products/240/ab240379/Images/ab240379-2-ab207777266346207777FC.jpg)
Flow Cytometry - Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)Flow cytometric analysis of 4% paraformaldehyde-fixed PC-12 (Rat adrenal gland pheochromocytoma cell line) cells labeling GCLC with ab207777 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207777).
-
![Immunoprecipitation - Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)](https://www.abcam.com/ps/products/240/ab240379/Images/ab240379-1-ab207777266345207777IP.jpg)
Immunoprecipitation - Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)GCLC was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab207777 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab207777 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: HepG2 whole cell lysate 10µg (Input).
Lane 2: ab207777 IP in HepG2 whole cell lysate.
Lane 3: Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab207777 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207777).
-
![Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)](https://www.abcam.com/ps/products/240/ab240379/Images/ab240379-5-benefits-of-recombinant-antibodies.png)
Anti-GCLC antibody [EPR20078] - BSA and Azide free (ab240379)
