Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22483-288] to CD8 alpha - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free
    See all CD8 alpha primary antibodies

  • Description

    Rabbit monoclonal [EPR22483-288] to CD8 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human colon and tonsil tissue. Flow Cyt: Human peripheral blood mononuclear cells. IP: CD8 alpha IP in human thymus lysate.

  • General notes

    Ab245830 is the carrier-free version of ab245118. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab245830 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD8 alpha with ab245118 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the T cell zone of human tonsil (PMID: 16504163) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245118).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD8 alpha with ab245118 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on lymphocytes of human colon (PMID: 26310568) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245118).
  • Flow Cytometry - Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)
    Flow Cytometry - Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)

    Flow cytometric analysis of human peripheral blood mononuclear cells (PBMC) labeling CD8 alpha with ab245118 at 1/400 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Dylight® 488, ab98462) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or anti-CD8 alpha RabMab (Right). Then stained with Alexa Fluor® 647-conjugated anti-CD4. The expressions of CD4 and CD8 alpha are mutually exclusive.

    Gated on viable lymphocytes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245118).

  • Immunoprecipitation - Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)
    Immunoprecipitation - Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)

    CD8 alpha was immunoprecipitated from 0.35 mg human thymus lysate with ab245118 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab245118 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: Human thymus lysate 10 µg (Input).
    Lane 2: ab245118 IP in human thymus lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245118 in human thymus lysate.

    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245118).

  • Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)
    Anti-CD8 alpha antibody [EPR22483-288] - BSA and Azide free (ab245830)

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