Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22682-21] to YB1 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-YB1 antibody [EPR22682-21] - BSA and Azide free
    See all YB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR22682-21] to YB1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Not recommended for Mouse IHC.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human mammary gland and breast cancer tissue. ICC/IF: MCF7 and NIH/3T3 cells. Flow cyt: MCF7 and A549 cells. IP: MCF7 cell lysate.

  • General notes

    Ab256516 is the carrier-free version of ab255606. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256516 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunoprecipitation - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)
    Immunoprecipitation - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)

    YB1 was immunoprecipitated from 0.35 mg MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10μg with ab255606 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab255606 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.


    Lane 1: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10μg
    Lane 2: ab255606 IP in MCF7 whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255606 in MCF7 whole cell lysate.


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.


    Exposure time: 30 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255606).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)

    Immunohistochemical analysis of paraffin-embedded Human mammary gland tissue labeling YB1 with ab255606 at 1/4000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human normal mammary gland (PMID: 21695211) is observed.  Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255606).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)

    Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling YB1 with ab255606 at 1/4000 dilution  followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in tumor cells of human breast cancer (PMID: 21695211) is observed. Counterstained with Hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255606).

  • Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)
    Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling YB1 with ab255606 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in NIH/3T3 cell line is observed. ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255606).

  • Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)
    Immunocytochemistry/ Immunofluorescence - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MCF7 (human breast adenocarcinoma epithelial cell) cells labelling YB1 with ab255606 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in MCF7 cell line is observed. ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255606).

  • Flow Cytometry - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)
    Flow Cytometry - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell) cells labelling YB1 with ab255606 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.  

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255606).
  • Flow Cytometry - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)
    Flow Cytometry - Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized A549 (Human lung carcinoma epithelial cell) cells labelling YB1 with ab255606 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.  

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255606).

  • Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)
    Anti-YB1 antibody [EPR22682-21] - BSA and Azide free (ab256516)

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