Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR11522(B)] to Ndufs1 - BSA and Azide free
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free
See all Ndufs1 primary antibodies
Description

Rabbit monoclonal [EPR11522(B)] to Ndufs1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
General notes
Ab249321 is the carrier-free version of ab157221. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249321 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR11522(B)
Isotype

IgG
Research areas

Western blot - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

All lanes : Anti-Ndufs1 antibody [EPR11522(B)] (ab157221) at 1/10000 dilution

Lane 1 : Raji cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : MOLT4 cell lysate
Lane 4 : Jurkat cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 79 kDa

This data was developed using ab157221, the same antibody clone in a different buffer formulation.
Immunocytochemistry - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of HepG2 cells labeling Ndufs1 with ab157221 at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.
ab157221 showing +ve staining in Human normal prostate. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.
ab157221 showing +ve staining in Human heart. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.ab157221 showing +ve staining in Human cervical carcinoma. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.ab157221 showing +ve staining in Human normal brain. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ndufs1 with ab157221 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ndufs1 with ab157221 at 1/100 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry - Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

This data was developed using ab157221, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized Jurkat cells labeling Ndufs1 with ab157221 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Anti-Ndufs1 antibody [EPR11522(B)] - BSA and Azide free (ab249321)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com