Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10595] to ARL 1 - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ARL 1 antibody [EPR10595] - BSA and Azide free
    See all ARL 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10595] to ARL 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249227 is the carrier-free version of ab155982. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249227 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Affinity purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10595

  • Isotype

    IgG

Images

  • Western blot - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Western blot - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    All lanes : Anti-ARL 1 antibody [EPR10595] (ab155982) at 1/1000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : MCF7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 20 kDa



    This data was developed using ab155982, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Immunocytochemistry - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)

    This data was developed using ab155982, the same antibody clone in a different buffer formulation.

    ICC/IF image of ab155982 stained CaCo2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab155982 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)

    This data was developed using ab155982, the same antibody clone in a different buffer formulation.

    ab155982 showing +ve staining in Human normal prostate. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)

    This data was developed using ab155982, the same antibody clone in a different buffer formulation.

    ab155982 showing +ve staining in Human gastric adenocarcinoma. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    This data was developed using ab155982, the same antibody clone in a different buffer formulation.ab155982 showing +ve staining in Human endometrial carcinoma. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    This data was developed using ab155982, the same antibody clone in a different buffer formulation.ab155982 showing +ve staining in Human normal brain. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    This data was developed using ab155982, the same antibody clone in a different buffer formulation.ab155982 showing +ve staining in Human normal liver. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    This data was developed using ab155982, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ARL 1 with ab155982 at 1/50 dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    This data was developed using ab155982, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling ARL 1 with ab155982 at 1/50 dilution Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)
    Anti-ARL 1 antibody [EPR10595] - BSA and Azide free (ab249227)

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