Anti-PLP2 antibody [EPR14238(B)] (ab180131)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14238(B)] to PLP2
- Suitable for: Flow Cyt, IP, WB, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-PLP2 antibody [EPR14238(B)]
See all PLP2 primary antibodies -
Description
Rabbit monoclonal [EPR14238(B)] to PLP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PLP2 aa 100 to the C-terminus. The exact sequence is proprietary.
Database link: Q04941 -
Positive control
- Human colon cancer and cerebellum lysates; SW480 cells; U87-MG cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14238(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PLP2 antibody [EPR14238(B)] (ab180131) at 1/5000 dilution
Lane 1 : Human colon cancer lysate
Lane 2 : Human cerebellum lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 17 kDa
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Immunofluorescence analysis of SW480 cells labeling PLP2 using ab180131 at 1/250 dilution. A Goat anti rabbit IgG (Dylight 488) at 1/250 dilution was used as secondary antibody. Cells were fixed with 4% paraformaldehyde. Counterstain: DAPI.
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Immunoprecipitation analysis of Human colon cancer lysate labeling PLP2 using ab180131 at 1/30 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 was used as secondary antibody.
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Flow cytometry analysis of U87-MG cells labeling PLP2 using ab180131 at 1/80 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.
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