Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 103 862 ₸

Order now and get it on Tuesday March 09, 2021

Anti-RPA32/RPA2 antibody [4E4] (ab252861)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [4E4] to RPA32/RPA2
Suitable for: WB, ICC/IF, ELISA
Reacts with: Mouse, Rat, Human

Product name

Anti-RPA32/RPA2 antibody [4E4]
See all RPA32/RPA2 primary antibodies
Description

Rabbit monoclonal [4E4] to RPA32/RPA2
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Human
ICC/IF	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HUVEC, U-2 OS, C6, NIH/3T3 cells Mouse, Rat and Human brain tissue, Human heart tissue; IF: HeLa cells; ELISA: Human RPA32/RPA2.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

4E4
Isotype

IgG1
Research areas

Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

All lanes : Anti-RPA32/RPA2 antibody [4E4] (ab252861) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell ), whole cell lysate
Lane 3 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 29 kDa

Blocking buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

Immunofluorescence analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 (green) with ab252861 at 1/50 dilution, followed by secondary antibody (ab150157) at 1:1000 (2µg/mL). Confocal image showing nuclear staining in HeLa cell line.

Counterstain: DAPI (blue) - ab179504 (YCA-R16775) Anti-beta IV Tubulin at 1:200 (2.5 µg/mL).

Counterstain Secondary antibody: ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594-Red) at 1:500 (20µg/mL).

Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary.
Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

All lanes : Anti-RPA32/RPA2 antibody [4E4] (ab252861) at 1/1000 dilution

Lane 1 : Human brain tissue lysate
Lane 2 : Human heart tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 29 kDa

Blocking buffer: 5% NFDM/TBST

Exposure time: 5 seconds
Western blot - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

All lanes : Anti-RPA32/RPA2 antibody [4E4] (ab252861) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/5000 dilution

Predicted band size: 29 kDa

Blocking buffer: 5% NFDM/TBST

Exposure time:

lane 1: 5.5 seconds
lane 2: 3 seconds
ELISA - Anti-RPA32/RPA2 antibody [4E4] (ab252861)

Human RPA32/RPA2= 1µg/mL

ab252861 used at 0-5000 ng/mL.

Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rat IgG (H+L) used at 1:1000.
Anti-RPA32/RPA2 antibody [4E4] (ab252861)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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