Human Anti-HSP60 IgG/A/M ELISA Kit (ab133059)
Key features and details
- Sensitivity: 2.88 ng/ml
- Range: 7.81 ng/ml - 250 ng/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Overview
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Product name
Human Anti-HSP60 IgG/A/M ELISA Kit
See all Hsp60 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 16 Inter-assay Sample n Mean SD CV% Overall 16 -
Sample type
Serum -
Assay type
Indirect -
Sensitivity
2.88 ng/ml -
Range
7.81 ng/ml - 250 ng/ml -
Assay time
1h 15m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human Anti-HSP60 IgG/A/M in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human Anti-HSP60 in Human Serum. This assay allows determination of IgG, IgA and IgM antibodies (total) to Human Hsp60 in serum.
A recombinant Human Hsp60 protein has been precoated onto 96-well plates. Standards or test samples are added to the wells, incubated and then washed. An anti-Human GAM-HRP conjugated antibody is then added and incubated. The plate is washed once more and the TMB substrate is then added which HRP catalyzes, generating a blue coloration after incubation. A stop solution is added which generates conversion to yellow color read at 450 nm which is proportional to the amount of analyte bound.
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Notes
HSP60 is a member of the chaperonin family of heat shock proteins, with homologs functioning in the cytosol and mitochondria to fold nascent and aggregated proteins. HSP60 is the eukaryotic homolog of the E. coli GroEL protein, and forms a multimeric complex in the mitochondria with Hsp10 (Cpn10) to form a large central cavity in which ATP-dependent protein folding takes place. TRiC/CCT, a eukaryotic relative of HSP60, is expressed in the cytosol and participates in the folding of actin and tubulin substrates, but lacks any association with an Hsp10-like co-factor.
Plates are not provided
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 100ml Anti-Human GAM-HRP Conjugate 1 x 10ml Human Anti-HSP60 IgG/A/M Microplate (12 x 8 wells) 1 unit Human Anti-HSP60 Standard 1 x 0.12ml Plate Sealer 3 units Sample Diluent 1 1 x 100ml Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Function
Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix. -
Involvement in disease
Defects in HSPD1 are a cause of spastic paraplegia autosomal dominant type 13 (SPG13) [MIM:605280]. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Defects in HSPD1 are the cause of leukodystrophy hypomyelinating type 4 (HLD4) [MIM:612233]; also called mitochondrial HSP60 chaperonopathy or MitCHAP-60 disease. HLD4 is a severe autosomal recessive hypomyelinating leukodystrophy. Clinically characterized by infantile-onset rotary nystagmus, progressive spastic paraplegia, neurologic regression, motor impairment, profound mental retardation. Death usually occurrs within the first two decades of life. -
Sequence similarities
Belongs to the chaperonin (HSP60) family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Alternative names
- 60 kDa chaperonin
- 60 kDa heat shock protein, mitochondrial
- CH60_HUMAN
see all -
Database links
- Entrez Gene: 3329 Human
- Omim: 118190 Human
- SwissProt: P10809 Human
- Unigene: 595053 Human
- Unigene: 727543 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab133059.