Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2876(2)] to RPA32/RPA2 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free
    See all RPA32/RPA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2876(2)] to RPA32/RPA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247754 is the carrier-free version of ab109084 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247754 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    Immunocytochemistry - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)

    This data was developed using ab109084, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:50 (9.34 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunocytochemistry - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    Immunocytochemistry - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)

    This data was developed using ab109084, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:50 (9.34 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    This data was developed using ab109084, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:460 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
  • Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    This data was developed using ab109084, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:460 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
  • Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    All lanes : Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HUVEC cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 29 kDa



    This data was developed using ab109084, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    This data was developed using ab109084, the same antibody clone in a different buffer formulation.ab109084 at 1/250 dilution staining RPA32/RPA2 in Human colon by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    This data was developed using ab109084, the same antibody clone in a different buffer formulation.ab109084 at 1/250 dilution staining RPA32/RPA2 in Human ovary carcinoma by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
    Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)

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