Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Wednesday March 10, 2021

Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22950-81] to RPA32/RPA2
Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
Reacts with: Human

Product name

Anti-RPA32/RPA2 antibody [EPR22950-81]
See all RPA32/RPA2 primary antibodies
Description

Rabbit monoclonal [EPR22950-81] to RPA32/RPA2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HeLa, HUVEC, U-2 OS whole cell lysate. Human brain tissue lysate. IHC-P: Human colon and glioma tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: HeLa cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22950-81
Isotype

IgG
Research areas

Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637) at 1/1000 dilution + Human brain tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 32 kDa
why is the actual band size different from the predicted?

Exposure time: 5.5 seconds.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

All lanes : Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HUVEC (human umbilical vein endothelial cell), whole cell lysate
Lane 3 : U-2 OS (human bone osteosarcoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Observed band size: 32 kDa why is the actual band size different from the predicted?

Exposure time: 5.5 seconds.

Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Immunoprecipitation - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

RPA32/RPA2 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab240637 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab240637 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg.

Lane 2: ab240637 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab240637 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 8 seconds.
Flow Cytometry - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

Flow cytometric analysis of 4% paraformaldehyde fixed. 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with ab240637 at 1/600 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with ab240637 at 1/100 dilution, followed by anti-RPA32/RPA2 ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing nuclear staining in HeLa cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling RPA32/RPA2 with ab240637 at 1/5000 dilution (0.103 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human glioma (PMID: 20154705) is observed. The section was incubated with ab240637 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling RPA32/RPA2 with ab240637 at 1/5000 dilution (0.103 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human colon (PMID: 20154705) is observed. The section was incubated with ab240637 for 10 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Anti-RPA32/RPA2 antibody [EPR22950-81] (ab240637)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com