Anti-Parkin antibody [PRK8] (ab77924)
![Anti-Parkin antibody [PRK8] (ab77924)](https://www.abcam.com/ps/products/77/ab77924/Images/ab77924-169148-ab77924GR1239510-WB.jpg "Anti-Parkin antibody [PRK8] (ab77924)")
Key features and details
- Mouse monoclonal [PRK8] to Parkin
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2b
Overview
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Product name
Anti-Parkin antibody [PRK8]
See all Parkin primary antibodies -
Description
Mouse monoclonal [PRK8] to Parkin -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB MouseRatHuman
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Immunogen
Recombinant full length protein corresponding to Human Parkin.
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Epitope
The epitope is the second ring domain (aa 399-465). -
Positive control
- WB: SH-SY5Y whole cell lysate. Human, mouse and rat brain tissue lysates. Wild-type HAP1 whole cell lysate. Flow Cyt: SH-SY5Y cells.
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General notes
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
PRK8 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Parkin antibody [PRK8] (ab77924)All lanes : Anti-Parkin antibody [PRK8] (ab77924) at 1/2000 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutesAll lanes blocked with 3% milk.
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![Flow Cytometry - Anti-Parkin antibody [PRK8] (ab77924)](https://www.abcam.com/ps/products/77/ab77924/Images/ab77924-261747-anti-parkin-antibody-prk8-flow-cytometry.jpg)
Flow Cytometry - Anti-Parkin antibody [PRK8] (ab77924)Overlay histogram showing SH-SY5Y cells stained with ab77924 (red line).
The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77924, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions.
Acquisition of >5,000 events was performed.
This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
