Anti-Parkin (phospho S101) antibody (ab73015)
Key features and details
- Rabbit polyclonal to Parkin (phospho S101)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Parkin (phospho S101) antibody
See all Parkin primary antibodies -
Description
Rabbit polyclonal to Parkin (phospho S101) -
Host species
Rabbit -
Specificity
ab73015 is specific for the ~52k parkin protein phosphorylated at Ser101. Immunolabeling of the parkin band is absent in parkin S101 mutants. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic phosphopeptide corresponding to amino acid residues surrounding phospho-Ser101 of human Parkin.
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Positive control
- HEK293 cells transfected with Parkin WT.
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General notes
Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab73015 is prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns. -
Primary antibody notes
Recent evidence suggests that phosphorylation of parkin at Ser101 may have an important regulatory role on its E3 ubiquitin ligase activity (Yamamoto et al., 2005). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Parkin (phospho S101) antibody (ab73015)All lanes : Anti-Parkin (phospho S101) antibody (ab73015) at 1/1000 dilution
Lane 1 : HEK293 cells transfected with Parkin WT (phospho)
Lane 2 : HEK293 cells transfected with Parkin S101 mutant (non-phospho)
Predicted band size: 52 kDa
Observed band size: 52 kDa
