Antibodies, Proteins, Kits and Reagents for Life Science

351 792 ₸ Product size

100 µl 351 792 ₸ 10 µl 144 067 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP13475] to GCLC
Suitable for: WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-GCLC antibody [EP13475]
See all GCLC primary antibodies
Description

Rabbit monoclonal [EP13475] to GCLC
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: NIH/3T3, Jurkat and C6 whole cell lysate. IP: Jurkat whole cell lysate.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP13475
Isotype

IgG
Research areas

Immunoprecipitation - Anti-GCLC antibody [EP13475] (ab190685)

ab190685 at 1/20 dilution immunoprecipitating GCLC in Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+): Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab190685 in Jurkat whole cell lysate
For western blotting, ab227648 at 1/500 dilution and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/50000 dilution were used.

Blocking and diluting buffer: 5% NFDM /TBST.
Western blot - Anti-GCLC antibody [EP13475] (ab190685)

Anti-GCLC antibody [EP13475] (ab190685) at 1/2000 dilution + NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 73 kDa

Blocking/diluting buffer and concentration: 5% NFDM/TBST

Exposure time: 180 seconds
Western blot - Anti-GCLC antibody [EP13475] (ab190685)

All lanes : Anti-GCLC antibody [EP13475] (ab190685) at 1/5000 dilution

Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 73 kDa

Blocking/diluting buffer and concentation: 5% NFDM/TBST

Exposure time lane 1: 80 seconds

Exposure time lane 2: 160 seconds
Anti-GCLC antibody [EP13475] (ab190685)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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