Anti-GCLC antibody (ab41463)
Key features and details
- Rabbit polyclonal to GCLC
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GCLC antibody
See all GCLC primary antibodies -
Description
Rabbit polyclonal to GCLC -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human GCLC aa 50-150 conjugated to keyhole limpet haemocyanin.
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Positive control
- This antibody gave a positive signal in the following Human Tissue Lysates: Liver, Kidney and Lung (Adenocarcinoma). This antibody gave a positive result in IHC in the following FFPE tissue: Human lung adenocarcinoma.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab41463 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatCowApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).Target
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Pathway
Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. -
Involvement in disease
Defects in GCLC are the cause of hemolytic anemia (HAGGSD) [MIM:230450]. -
Sequence similarities
Belongs to the glutamate--cysteine ligase type 3 family. - Information by UniProt
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Database links
- Entrez Gene: 2729 Human
- Entrez Gene: 14629 Mouse
- Entrez Gene: 25283 Rat
- Omim: 606857 Human
- SwissProt: P48506 Human
- SwissProt: P97494 Mouse
- SwissProt: P19468 Rat
- Unigene: 654465 Human
see all -
Alternative names
- EC 6.3.2.2 antibody
- Gamma ECS antibody
- Gamma glutamylcysteine synthetase antibody
see all
Images
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Western blot - Anti-GCLC antibody (ab41463)All lanes : Anti-GCLC antibody (ab41463) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Human lung tissue lysate (adenocarcinoma) - insoluble fraction (female, 80 years) (ab42923)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Additional bands at: 58 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
References (25)
ab41463 has been referenced in 25 publications.
- Tang X et al. Blockade of Glutathione Metabolism in IDH1-Mutated Glioma. Mol Cancer Ther 19:221-230 (2020). PubMed: 31548295
- Matsuda Y et al. NFE2L2 activator RS9 protects against corneal epithelial cell damage in dry eye models. PLoS One 15:e0229421 (2020). PubMed: 32320433
- Rajasekaran NS et al. Reductive stress impairs myogenic differentiation. Redox Biol 34:101492 (2020). PubMed: 32361680
- Song CH et al. Effects of 17ß-estradiol on colorectal cancer development after azoxymethane/dextran sulfate sodium treatment of ovariectomized mice. Biochem Pharmacol 164:139-151 (2019). PubMed: 30981879
- Cho SS et al. Cudrania Tricuspidata Extract and Its Major Constituents Inhibit Oxidative Stress-Induced Liver Injury. J Med Food 22:602-613 (2019). PubMed: 31045470
Images
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Western blot - Anti-GCLC antibody (ab41463)All lanes : Anti-GCLC antibody (ab41463) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : Human lung tissue lysate (adenocarcinoma) - insoluble fraction (female, 80 years) (ab42923)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : Human liver tissue lysate - total protein (ab29889)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Additional bands at: 58 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
