Anti-ACADS/SCAD antibody (ab79169)
Key features and details
- Rabbit polyclonal to ACADS/SCAD
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACADS/SCAD antibody
See all ACADS/SCAD primary antibodies -
Description
Rabbit polyclonal to ACADS/SCAD -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Chicken, Cow, Pig, Orangutan
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Immunogen
Synthetic peptide corresponding to Human ACADS/SCAD aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab91440) -
Positive control
- Recombinant Human ACADS/SCAD protein (ab95383) can be used as a positive control in WB. This antibody gave a positive signal in the following human tissue lysates: liver; heart; skeletal muscle.
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General notes
Previously labelled as ACADS.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab79169 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Pathway
Lipid metabolism; mitochondrial fatty acid beta-oxidation. -
Involvement in disease
Defects in ACADS are the cause of acyl-CoA dehydrogenase short-chain deficiency (ACADSD) [MIM:201470]. It is an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants, and a form of lipid-storage myopathy in adults. -
Sequence similarities
Belongs to the acyl-CoA dehydrogenase family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 35 Human
- Entrez Gene: 64304 Rat
- Omim: 606885 Human
- SwissProt: P16219 Human
- SwissProt: P15651 Rat
- Unigene: 507076 Human
- Unigene: 1167 Rat
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Alternative names
- ACAD3 antibody
- ACADS antibody
- ACADS_HUMAN antibody
see all
Images
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Western blot - Anti-ACADS/SCAD antibody (ab79169)All lanes : Anti-ACADS/SCAD antibody (ab79169) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-ACADS/SCAD antibody (ab79169)ICC/IF image of ab79169 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79169, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
Protocols
Datasheets and documents
References (0)
ab79169 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-ACADS/SCAD antibody (ab79169)All lanes : Anti-ACADS/SCAD antibody (ab79169) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 44 kDa
Additional bands at: 68 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
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Immunocytochemistry/ Immunofluorescence - Anti-ACADS/SCAD antibody (ab79169)ICC/IF image of ab79169 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79169, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.
