Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10862(B)] to ACADS/SCAD
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ACADS/SCAD antibody [EPR10862(B)]
    See all ACADS/SCAD primary antibodies

  • Description

    Rabbit monoclonal [EPR10862(B)] to ACADS/SCAD

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    corresponding to Human ACADS/SCAD.
    Database link: P16219

  • Positive control

    • WB: HeLa, Mouse liver, and Rat kidney lysates. IHC-P: human hepatocellular carcinoma, Human heart and Human thyroid gland tissues tissue. IP: Human liver cells.

  • General notes

     

     

    Previously labelled as ACADS. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue sections labeling ACADS/SCAD with Purified ab156571 at 1:100 dilution (3.76 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Western blot - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Western blot - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    All lanes : Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571) at 1/1000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : Mouse liver lysates
    Lane 3 : Rat kidney lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 44 kDa
    Observed band size: 41 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Immunoprecipitation - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    ab156571 (Purified) at 1:20 dilution (2 µg) immunoprecipitating ACADS/SCAD in Human liver lysate.
    Lane 1 (input): Human liver lysate 10 µg
    Lane 2 (+): ab156571 & Human liver lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab156571 in Human liver lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)

    ab156571 showing +ve staining in Human normal liver.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)

    ab156571 showing +ve staining in Human gastric adenocarcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)

    ab156571 showing +ve staining in Human normal colon.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)

    ab156571 showing +ve staining in Human urinary bladder carcinoma.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)

    Immunohistochemical analysis of paraffin embedded Human heart tissue labeling ACADS/SCAD with ab156571 antibody at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)

    Immunohistochemical analysis of paraffin embedded Human thyroid gland tissue labeling ACADS/SCAD with ab156571 antibody at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)
    Anti-ACADS/SCAD antibody [EPR10862(B)] (ab156571)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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