Anti-Histone H3 (mono methyl K56) antibody (ab66857)
Key features and details
- Rabbit polyclonal to Histone H3 (mono methyl K56)
- Suitable for: ICC/IF, WB
- Reacts with: Cow, Human
- Isotype: IgG
Overview
-
Product name
Anti-Histone H3 (mono methyl K56) antibody
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (mono methyl K56) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Pig, Drosophila melanogaster, Zebrafish, Chinese hamster
-
Immunogen
-
Positive control
- This antibody gave a positive signal in Calf Thymus Histone lysate as well as the following whole cell lysates: HeLa; HeLa Nuclear; HeLa Histone Nuclear. This antibody gave a positive result when used in the following methanol fixed cell lines: MCF-7 and HepG2.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Histone H3 (mono methyl K56) antibody (ab66857)
ab66857 stained MCF-7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab66857 at 1µg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit DyLight® 488 (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in Methanol fixed (100%) HepG2 cells.
-
Western blot - Anti-Histone H3 (mono methyl K56) antibody (ab66857)All lanes : Anti-Histone H3 (mono methyl K56) antibody (ab66857) at 1 µg/ml
Lane 1 : Calf Thymus Histone Preparation Nuclear Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 : HeLa Histone Preparation Nuclear Lysate
Lane 5 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K56) peptide (ab150460) at 1 µg/ml
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Histone H3 (mono methyl K56) peptide (ab150460) at 1 µg/ml
Lane 7 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with Human Histone H3 (mono methyl K56) peptide (ab150460) at 1 µg/ml
Lane 8 : HeLa Histone Preparation Nuclear Lysate with Human Histone H3 (mono methyl K56) peptide (ab150460) at 1 µg/ml
Lane 9 : Calf Thymus Histone Preparation Nuclear Lysate with Human Histone H3 peptide (ab172484) at 1 µg/ml
Lane 10 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human Histone H3 peptide (ab172484) at 1 µg/ml
Lane 11 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate with Human Histone H3 peptide (ab172484) at 1 µg/ml
Lane 12 : HeLa Histone Preparation Nuclear Lysate with Human Histone H3 peptide (ab172484) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 14 kDa, 23 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab66857 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution.
