Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab195489)
Key features and details
- Rabbit polyclonal to Histone H3 (tri methyl K36) - ChIP Grade
- Suitable for: WB, Dot blot, ChIP, ChIP-sequencing
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade
See all Histone H3 primary antibodies -
Description
Rabbit polyclonal to Histone H3 (tri methyl K36) - ChIP Grade -
Host species
Rabbit -
Tested applications
Suitable for: WB, Dot blot, ChIP, ChIP-sequencingmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Drosophila melanogaster, Zebrafish
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Immunogen
Synthetic peptide within Human Histone H3 (tri methyl K36) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
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Positive control
- Chromatin prepared from HeLa and HeLaS3 cells; HeLa histone extract.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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ChIP-sequencing - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab195489)
ChIP was performed with 2 µg of ab195489 against H3 (tri methyl K36) on sheared chromatin from 1 million HeLaS3 cells ans described. The IP’d DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer’s instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm.
Figure shows the obtained profiles in genomic regions of chromosome 12 (including the GAPDH positive control), 7 (including the ACTB positive control), 14 and 3, respectively. These results clearly show an enrichment of the H3K36me3 at active genes.
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ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab195489)ChIP-seq results obtained with ab195489 directed against H3 (tri methyl K36).
ChIP was performed with 2 µg of ab195489 against H3 (tri methyl K36) on sheared chromatin from 1 million HeLaS3 cells. IgG (2 µg/IP) was used as a negative IP control. The IP’d DNA was analysed by QPCR with optimized PCR primer pairs for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the coding region of the active ACTB gene.
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Western blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab195489)Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab195489) at 1/1000 dilution + HeLa histone extract at 15 µg
Predicted band size: 15 kDaAntibody diluted in TBS-Tween containing 5% skimmed milk.
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Dot Blot - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab195489)Dot Blot analysis was performed to test the cross reactivity of ab195489 against Histone H3 (tri methyl K36) with peptides containing other H3 and H4 modifications and the unmodified sequence. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane. ab195489 was used at a dilution of 1/20,000. Figure shows a high specificity of the antibody for the modification of interest.
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ChIP - Anti-Histone H3 (tri methyl K36) antibody - ChIP Grade (ab195489)ChIP results obtained with ab195489 directed against H3 (tri methyl K36).
ChIP assays were performed using HeLa cells, ab195489 and optimized PCR primer sets for qPCR. ChIP was performed using sheared chromatin from 1 million cells. A titration consisting of 1, 2, 5 and 10 µg of antibody per ChIP experiment was analyzed. IgG (2 µg/IP) was used as a negative IP control. QPCR was performed with primers for the promoter and coding region of the active GAPDH, for a region located 1 kb upstream of the GAPDH promoter and for the Sat2 satellite repeat. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
