Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)
Key features and details
- Rabbit polyclonal to Histone H4 (symmetric di methyl R3)
- Suitable for: IHC-P, PepArr, WB
- Reacts with: Cow, Human, Arabidopsis thaliana
- Isotype: IgG
Overview
-
Product name
Anti-Histone H4 (symmetric di methyl R3) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (symmetric di methyl R3) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Cow
-
Immunogen
Synthetic peptide within Histone H4 aa 1-100 (symmetric di methyl R3) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available asab14791) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Images
-
Western blot - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823) at 1 µg + Calf Thymus Histone Preparation Nuclear Lysate at 0.5 µg
Secondary
Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11.5 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab5823 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)
IHC image of ab5823 staining Histone H4 (symmetric di methyl R3) in human kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5823, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
-
Peptide Array - Anti-Histone H4 (symmetric di methyl R3) antibody (ab5823)All batches of ab5823 are tested in Peptide Array against peptides to different Histone H4 and H2A modifications. Six dilutions of each peptide are printed on to the Peptide Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to Histone H4 - symmetric di methyl R3 peptide (ab14791), indicating that this antibody specifically recognises the Histone H4 - symmetric di methyl R3 modification.
ab14791 - Histone H4 - symmetric di methyl R3
ab17416 - Histone H4 asymmetric di methyl R3
ab15821 - Histone H4 - unmodified
ab22399 - Histone H2A symmetric di methyl R3
ab22398 - Histone H2A asymmetric di methyl R3
ab13186 - Histone H2A - unmodified
