Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP852Y] to Glycogen synthase 1/GYS1 (phospho S641) - BSA and Azide free
  • Suitable for: IHC-P, WB, Dot blot
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free
    See all Glycogen synthase 1/GYS1 primary antibodies

  • Description

    Rabbit monoclonal [EP852Y] to Glycogen synthase 1/GYS1 (phospho S641) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Detects Glycogen synthase 1/GYS1 only when phosphorylated on Serine 641.

  • Tested applications

    Suitable for: IHC-P, WB, Dot blotmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247514 is the carrier-free version of ab81230 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247514 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. This product was previously labelled as Glycogen synthase 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)
    Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)
    All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230) at 1/2000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) treated with insulin at 20 ug/mL for 15 minutes. Whole cell lysate
    Lane 2 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate.Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa


    Exposure time: 1 second


    This data was developed using ab81230, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)
    Western blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)
    All lanes : Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] (ab81230) at 1/10000 dilution

    Lane 1 : Mouse muscle tissue lysate, untreated
    Lane 2 : Mouse muscle tissue lysate, treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa



    This data was developed using ab81230, the same antibody clone in a different buffer formulation.

  • Dot Blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)
    Dot Blot - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)
    This data was developed using ab81230, the same antibody clone in a different buffer formulation.Dot blot analysis of glycogen synthase 1/GYS1 (phospho S641) phospho peptide (Lane 1) and glycogen synthase 1/GYS1 non-phospho peptide (Lane 2) labeling synthase 1 (phospho S641) with ab81230 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody. Blocking and diluting buffer: 5% NFDM/TBST. Exposure time: 3 minutes.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)
    This data was developed using ab81230, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Human liver tissue, using 1/100 ab81230. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)
    Anti-Glycogen synthase 1/GYS1 (phospho S641) antibody [EP852Y] - BSA and Azide free (ab247514)

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