Anti-NPM1/ALK antibody [EPR11413] - BSA and Azide free (ab250246)
![Anti-NPM1/ALK antibody [EPR11413] - BSA and Azide free (ab250246)](https://www.abcam.com/ps/products/250/ab250246/Images/ab250246-1-anti-npm1-alk-antibody-epr11413-immunohistochemistry-anaplastic-large-cell-lymphoma-human.jpg "Anti-NPM1/ALK antibody [EPR11413] - BSA and Azide free (ab250246)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11413] to NPM1/ALK - BSA and Azide free
- Suitable for: IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-NPM1/ALK antibody [EPR11413] - BSA and Azide free
See all NPM1/ALK primary antibodies -
Description
Rabbit monoclonal [EPR11413] to NPM1/ALK - BSA and Azide free -
Host species
Rabbit -
Specificity
In more than 50% of systemic Anaplastic large cell lymphoma, t(2;5)(p23;q35) translocation can be found (Falini et al., 1999; Morris et al., 2001). (PMID: 9736036 and PMID: 11380391). The corresponding genes have been cloned and represent a fusion between the NPM and the ALK genes (Morris et al., 1994). (PMID: 8122112). Encodes a chimeric 80-kDa protein consisting of the N-terminal portion (amino acids 1-117) of NPM fused to the cytoplasmic portion of ALK (amino acids 1058-1620). Reference: Galietta et al., 2007. (PMID: 17537995).
-
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
-
Immunogen
Fusion protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- IHC-P: Human anaplastic large cell lymphoma tissue
-
General notes
Ab250246 is the carrier-free version of ab180607. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250246 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11413 -
Isotype
IgG -
Research areas
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NPM1/ALK antibody [EPR11413] - BSA and Azide free (ab250246)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human anaplastic large cell lymphoma tissue sections labeling NPM1/ALK with purified ab180607 at 1/16,000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180607) -
![Anti-NPM1/ALK antibody [EPR11413] - BSA and Azide free (ab250246)](https://www.abcam.com/ps/products/250/ab250246/Images/ab250246-2-benefits-of-recombinant-antibodies.png)
Anti-NPM1/ALK antibody [EPR11413] - BSA and Azide free (ab250246)
