Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7769] to MMP22 - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-MMP22 antibody [EPR7769] - BSA and Azide free
    See all MMP22 primary antibodies

  • Description

    Rabbit monoclonal [EPR7769] to MMP22 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250359 is the carrier-free version of ab181118. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250359 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)
    This data was developed using ab181118, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human testis tissue labeling MMP22 with ab181118 at 1/250. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)
    Immunocytochemistry - Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)
    This data was developed using ab181118, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling MMP22 with ab181118 at 1/100 followed by fluorescence labelled Goat anti-rabbit IgG at 1/200 and counterstained with Dapi.
  • Western blot - Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)
    Western blot - Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)
    All lanes : Anti-MMP22 antibody [EPR7769] (ab181118) at 1/1000 dilution

    Lane 1 : Calu 3 cell lysate
    Lane 2 : SK OV 3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 44 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab181118, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)
    Western blot - Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)
    Anti-MMP22 antibody [EPR7769] (ab181118) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size: 44 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    This data was developed using ab181118, the same antibody clone in a different buffer formulation.

     

  • Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)
    Anti-MMP22 antibody [EPR7769] - BSA and Azide free (ab250359)

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