Glutamate Assay Kit (Fluorometric) (ab138883)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue, Urine
- Sensitivity: 1 µM
Overview
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Product name
Glutamate Assay Kit (Fluorometric)
See all Glutamate kits -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Tissue, Cell Lysate -
Assay type
Quantitative -
Sensitivity
1 µM -
Assay time
0h 30m -
Product overview
Glutamate Assay Kit (Fluorometric) (ab138883) provides a quick and sensitive method for the measurement of glutamate (glutamic acid) in various biological samples.
In the glutamate assay protocol, the coupled enzyme system catalyzes the reaction between L-Glutamic acid and NADP+ to produce NADPH, which is specifically recognized by the NADPH sensor and recycled back to NADP+. During the reaction, a red fluorescence product is produced, which in turn can be detected in a fluorescence microplate reader at Ex/Em = 540/590 nm (range Ex/Em = 530 – 570/590 – 600 nm).
This assay can detect as little as 1 µM glutamic acid. The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.
The assay is robust, and can be easily adapted to automation without separation step as no wash step is required.
Glutamate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min - 2 hr
- analyze with a microplate reader -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 10ml Dilution Buffer 1 x 10ml Enzyme Mix 1 unit Glutamic Acid 1 vial NADP 1 vial -
Research areas
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Relevance
Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry.
Images
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Intracellular glutamate was measured using ab138883 Amrani A et al., PLoS One, 9(9). Fig 5a. doi: 10.1371/journal.pone.0106831 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
The concentration of intracellular glutamate in cells, during the exponential phase, grown at different hydrostatic pressures.
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Functional Assay: Glutamate Assay Kit (fluorometric) (ab138883)Standard curve (30 minutes incubation): mean of duplicates (+/- SD), with background reads subtracted.
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Functional Assay: Glutamate Assay Kit (fluorometric) (ab138883)Glutamic acid measured in mouse tissue lysates, showing quantity (nmol) per mg of extracted protein (mean of duplicates, +/- SD).
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Functional Assay: Glutamate Assay Kit (fluorometric) (ab138883)Glutamic acid measured in cell lysates showing quantity (nmol) per 106 cells (mean of duplicates, +/- SD).
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Functional Assay: Glutamate Assay Kit (fluorometric) (ab138883)Glutamate measured in human biological fluids (mean of duplicates, +/- SD).
